Product Description

PGLS Polyclonal Antibody

Catalogue Numbers: BS5852-50, BS5852-100

Sizes: 50µl, 100µl

Alternative Name: 6-phosphogluconolactonase; 6PGL

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O95336

Host: Rabbit

Reactivity: Human

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: PGLS (6-phosphogluconolactonase), also known as 6PGL, is a 258 amino acid protein that belongs to the glucosamine/galactosamine-6-phosphate isomerase family and the 6-phosphogluconolactonase subfamily. Localizing to cytoplasm, PGLS is a particularly active enzyme that catalyzes the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate, which is the second step of the pentose phosphate pathway. Highly conserved, PGLS shares 33% to 37% sequence similarity with yeast Sol1, Sol2, Sol3 and Sol4, 26% similarity with the C-terminal portion of human H6PD, 20% to 25% similarity with bacterial devB proteins and 17% similarity with human GNPDA1. PGLS erythrocyte deficiency, an autosomal dominant disorder, in conjunction with G6PD deficiency, may play a role in hemolytic anemia.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PGLS polyclonal antibody detects endogenous levels of PGLS protein.

Bioworld Molecular Weight: ~ 28 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PGLS (A226) pAb at 1:500 dilution
Lane 1: HCT116 whole cell lysate(40ug)
Lane 2: Panc1 whole cell lysate(40ug)
Lane 3: HEK293T whole cell lysate(40ug)

Immunogen: Synthetic peptide, corresponding to amino acids 213-258 of Human PGLS.

Conjugate: Unconjugated

Modification: Unmodified