Product Description

PGP9.5 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9416-50, MB9416-100

Product: Mouse IgG2a, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P09936(Human) Q9R0P9(Mouse) Q00981(Rat)

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IHC ICC FC

All Applications: WB:1:500
ICC:1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Purification and Purity: Peptide affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: PGP9.5 monoclonal antibody detects endogenous levels of PGP9.5 protein.

Bioworld Molecular Weight: 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PGP9.5 on A172 (1) and SHG-44 (2) using anti-PGP9.5 antibody at 1/500 dilution., ICC staining PGP9.5 (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: UCH-L1, PGP9.5, UCHL1, Epididymis luminal protein 117, Epididymis secretory protein Li 53, HEL 117, HEL S 53, NDGOA, Neuron cytoplasmic protein 9.5, OTTHUMP00000218137, OTTHUMP00000218139, OTTHUMP00000218140, OTTHUMP00000218141, Park 5, PARK5, PGP 9.5, PGP9.5, PGP95, Protein gene product 9.5, Ubiquitin C terminal esterase L1, Ubiquitin C terminal hydrolase, Ubiquitin C terminal hydrolase L1, Ubiquitin carboxyl terminal esterase L1, Ubiquitin carboxyl terminal hydrolase isozyme L1, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Ubiquitin thioesterase L1, Ubiquitin thiolesterase, Ubiquitin thiolesterase L1, UCH-L1, UCHL1, UCHL1,

Immunogen: Synthetic peptide within C-terminal human PGP9.5.

Conjugate: Unconjugated

Modification: Unmodified