Product Description

PHAX Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: RA28722-50, RA28722-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: function:A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA., PTM:Phosphorylated in the nucleus. Dephosphorylated in the cytoplasm (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR., PTM:Phosphorylated upon DNA damage, probably by ATM or ATR., similarity:Belongs to the PHAX family., subcellular location:Located in the nucleoplasm and Cajal bodies. Shuttles between the nucleus and the cytoplasm. Shuttles between the nucleoplasm and Cajal bodies., subunit:Found in a U snRNA export complex with PHAX/RNUXA, NCBP1, NCBP2, RANPO1 and m7G-capped RNA. Part of a precomplex with PHAX/RNUXA, NCBP1, NCBP2 and m7G-capped RNA. Interacts with NCBP1 (By similarity). Found in a complex with snoRNA.,

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB, ELISA

Alternate Names: PHAX, RNUXA, Phosphorylated adapter RNA export protein, RNA U small nuclear RNA export adapter protein

Clonality: Polyclonal

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight: 48

Protein Molecular Weight (kDa): 48

Storage: -20°C/1 year

Human Gene ID: 51808

Human SwissProt Number: Q9H814

Condition: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Research Use Only