PHB Rabbit Monoclonal Antibody-BS9857M

PHB Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9857M-50, BS9857M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P35232

Host: Rabbit

Reactivity: Human, Mouse, Rat, Zebra fish

Applications: WB, ICC, IHC, IP, FC

All Applications: WB: 1:1000-1:2000
ICC: 1:50-1:200
IHC: 1:200-1:500
FC: 1:50-1:100

Background: Prohibitin is an evolutionarily conserved protein that has antiproliferative activity. The gene encoding human prohibitin maps to chromosome 17q21 and is ubiquitously expressed. Prohibitin is a post-synthetically modified protein that is localized in the inner membrane of mitochondria, where it regulates the cell cycle by blocking the transition between the G1 and S phases, and on the plasma membrane of B cells, where it mediates B cell maturation.

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of PHB and does not cross-react with related proteins.

Bioworld Molecular Weight: ~ 30 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PHB Rabbit mAb at 1:1000 dilution
Lane 1: 293 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: The kidney tissue lysate of Mouse
Lane 4: HepG2 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PHB antibody. Counter stained with hematoxylin., ICC staining Prohibitin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS., Flow cytometric analysis of HepG2 cells with PHB antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Alternative Name: Prohibitin

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Unmodified