Product Description

PHD1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB12321-50, MB12321-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q96KS0

Host: Rabbit

Reactivity: Human, Rat

Applications: WB,

All Applications: WB: 1/500-1/1000

Background: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 44 kDa; Observed MW: 44 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PHD1/prolyl hydroxylase in rat heart lysates using PHD1 antibody., Western blot analysis of PHD1/prolyl hydroxylase in Hela, A549, HL-60, U2OS, U87-MG lysates using PHD1/prolyl hydroxylase antibody.

Alternative Name: Estrogen-induced tag 6; HPH-3; PHD1

Immunogen: A synthesized peptide derived from human PHD1/prolyl hydroxylase

Conjugate: Unconjugated

Modification: Unmodified