PHF19 Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-62410-60, E-AB-62410-120, E-AB-62410-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: PHF19
Target Synonym: PHF19; MTF2L1; PCL3; TDRD19B
Research Areas: Epigenetics and Nuclear Signaling
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IF
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: Q5T6S3
Background: Polycomb group (PcG protein that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3 and recruits the PRC2 complex, thus enhancing PRC2 H3K27me3 methylation activity. Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases RIOX1 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing. Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds histone H3 dimethylated at 'Lys-36' (H3K36me2. Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter.
Concentration: 1 mg/mL
Immunogen: Recombinant fusion protein of human PHF19
Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3.
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000, IF 1:50-1:100
Calculated MW: 14kDa/22kDa/65kDa
Observed MW: 66kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only