PHF8 Polyclonal Antibody-BS91068

PHF8 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91068-50, BS91068-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9UPP1(Human)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: PHF8 polyclonal antibody detects endogenous levels of PHF8 protein.

Bioworld Molecular Weight: 118 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PHF8 on different cell lysate using anti-PHF8 antibody at 1/500 dilution.
Positive control:
Lane 1: PC-3M
Lane 2: A431
Lane 3: Human kidney tissue, ICC staining PHF8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: PHF8, KIAA1111, ZNF422, Histone lysine demethylase PHF8, JHDM1F, Jumonji C domain containing histone demethylase 1F, MRXSSD, PHD finger protein 8, PHF8, PHF8, ZNF422,

Immunogen: Recombinant protein within human PHF8 aa 150-350.

Conjugate: Unconjugated

Modification: Unmodified