Product Description

PHGDH Mouse Monoclonal Antibody

Sizes: 25μl, 100μl

Catalogue Numbers: MB4038-25, MB4038-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Mammalian cells synthesize serine de novo by diverting a portion of the glycolytic intermediate 3-phosphoglycerate into the phosphorylated pathway of serine synthesis. This shift supports anabolism by providing precursors for the biosynthesis of proteins, nucleotides, creatine, porphyrins, phospholipids, and glutathione. Phosphoglycerate dehydrogenase (PHGDH) catalyzes the first step in the serine biosynthesis pathway by converting 3-phosphoglycerate into phosphohydroxy pyruvate. Research studies demonstrate that an increase in serine biosynthesis supports growth and proliferation of cancer cells, which is supported by amplification and overexpression of PHGDH in a subset of melanoma and breast cancers. Suppression of PHGDH expression in cell lines with elevated PHGDH levels causes a strong decrease in cell proliferation and inhibits tumor growth in vivo. Additional evidence suggests that PHGDH interacts with and stabilizes FoxM1, which promotes the proliferation, invasion, and tumorigenicity of glioma cells.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Mouse

Applications: IHC, WB

Alternate Names: D-3-phosphoglycerate dehydrogenase, 3-PGDH, 2-oxoglutarate reductase, Malate dehydrogenase, PHGDH, PGDH3

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: Fμll length human recombinant protein of human PHGDH (NP_006614) produced in HEK293T cell.

Dilution: WB 1:2000, IHC 1:150

Purification: The antibody was affinity-purified from mouse ascites fluids or tissue cμlture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: IgG2a

Molecular Weight: ~ 57 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O43175

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only