Product Description

PHKB Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS5854-50, BS5854-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Phosphorylase kinase is a hexadecameric enzyme that is comprised of four copies of four subunits that are encoded by four separate genes: PHKA, PHKB, PHKG, and PHKD. This serine/threonine specific kinase converts glycogen phosphorylase b to glycogen phosphorylase a, resμlting in the release of glucose-1-phophate from glycogen. PHKB (Phosphorylase b kinase regμlatory subunit beta) is a 1093 amino acid subunit of phosphorylase kinase that, along with PHKA, has regμlatory functions controlled by phosphorylation. Defects in the gene encoding PHKB are the cause of glycogen storage disease type 9B, which is also known as phosphorylase kinase deficiency of liver and muscle. This disease is characterized by a mild phenotype of hepatomegaly with only slightly elevated transaminase and plasma lipids, no clinical muscle involvement, and generally is correlated with a gradual improvement with increasing age. There are four isoforms of PHKB that are produced as a resμlt of alternative splicing events.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: Phosphorylase b kinase regμlatory subunit beta, Phosphorylase kinase subunit beta, Phosphorylase B

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 699-744 of Human PHKB.

Dilution: WB: 1:500~1:1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: PHKB polyclonal antibody detects endogenous levels of PHKB protein.

Molecular Weight: ~ 125 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q93100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only