PHKG1 Polyclonal Antibody-BS5855

PHKG1 Polyclonal Antibody

Catalogue Numbers: BS5855-50, BS5855-100

Sizes: 50µl, 100µl

Alternative Name: Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform; PHK-gamma-M; Phosphorylase kinase subunit gamma-1; Serine/threonine-protein kinase PHKG1; PHKG; PHK-γ-M; PHKγ-M; PHK-γM; PHKγM;

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16816

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB ICC

All Applications: WB: 1:500~1:1000
ICC: 1:50~1:200

Background: PHKG1 (phosphorylase kinase subunit γ1), is a subunit of phosphorylase kinase (PHK) that belongs to the Ser/Thr protein kinase family. PHK is a hexadecameric protein composed of four α chains, four β chains, four γ chains and four δ chains. The γ chains are catalytic chains, the α and β chains are regulatory chains and the δ chains are calmodulins. PHKG1 contains two calmodulin-binding domains and one protein kinase domain. As the catalytic chain of PHK, PHKG1 is responsible for catalyzing the phosphorylation and activation of glycogen phosphorylase and therefore it plays an important role in the glycogenolytic pathway. Mutations in the gene encoding PHKG1 can lead to PHK deficiency and result in glycogen storage disease type 9C (GSD9C), also known as autosomal liver glycogenosis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: PHKG1 polyclonal antibody detects endogenous levels of PHKG1 protein.

Bioworld Molecular Weight: ~ 45 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of PHKG1 polyclonal antibody at 1:500 dilution
Lane 1: A549 cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: Rat brain tissue lysate

Immunogen: Synthetic peptide, corresponding to amino acids 293-338 of Human PHKG1.

Conjugate: Unconjugated

Modification: Unmodified