PHLDA3 rabbit pAb - ES3194

PHLDA3 rabbit pAb

Sizes: 50μL, 100μL

Catalogue Numbers: ES3194-50, ES3194-100

Citations, Manuals and MSDS Available upon request.

Background: domain:The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4, 5-bisphosphate (PIP2) and phosphatidylinositol 3, 4, 5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling., function:p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor., induction:By p53/TP53; expression is directly activated by p53/TP53. p53/TP53 phosphorylation on 'Ser-15' is required to activate the PHLDA3 promoter., miscellaneous:PHLDA3 genomic locus is frequently observed in primary lung cancers, suggesting a role in tumor suppression., similarity:Belongs to the PHLDA3 family., similarity:Contains 1 PH domain., tissue specificity:Widely expressed with lowest expression in liver and spleen.,

Alternate Name: PHLDA3; TIH1; Pleckstrin homology-like domain family A member 3; TDAG51/Ipl homolog 1

Source: Rabbit

Applications: WB; IHC; IF; ELISA

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Reactivity: Human; Mouse; Rat

Immunogen: The antiserum was produced against synthesized peptide derived from human PHLDA3. AA range:21-70

Storage and Stability: -20°C/1 year

Clonality: Polyclonal

Isotype: IgG

Concentration: 1 mg/ml

Observed Band (KD): 15kD

Human Gene ID: 23612

Human SWISS Prot NO: Q9Y5J5

Subcellular Location: Cytoplasm. Membrane; Peripheral membrane protein.

Research Use Only