Product Description

Phospho-4E BP1 (Thr37) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16301-50, BZ16301-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q13541

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100

Background: Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 13 kDa; Observed MW: 18 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-4E BP1 in MDAMB435 lysates using Phospho-4E BP1 antibody., Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma, using 4EBP1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval. Sample with blocking peptide on the right.

Alternative Name: EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I

Immunogen: Synthetic peptide of human EIF4EBP1

Conjugate: Unconjugated

Modification: Phosphorylated