Phospho-AKT (Ser473) (8A9) Monoclonal Antibody-MB3262

Phospho-AKT (Ser473) (8A9) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB3262-50, MB3262-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P31749

Host: Mouse

Reactivity: Human

Applications: WB, IHC-P

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100

Background: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG1

Bioworld Molecular Weight: Calculated MW: 56 kDa; Observed MW: 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-AKT in PC-3 lysates using Phospho-AKT antibody., Immunohistochemistry analysis of paraffin-embedded Human Lung Carcinoma Tissue using Phospho-Akt Ser473 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Alternative Name: AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha

Immunogen: Synthetic peptide conjugated to KLH.

Conjugate: Unconjugated

Modification: Phosphorylated