Phospho-AKT (Ser473) Monoclonal Antibody-MB10435

Phospho-AKT (Ser473) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10435-50, MB10435-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: P31749

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1/500-1/1000

Background: Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-AKT in C6, 3T3, HelaLysates using Phospho-AKT antibody.

Bioworld Molecular Weight: Calculated MW: 56 kDa; Observed MW: 56 kDa

Specificity: IgG

Alternative Name: AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser473 of human AKT1

Conjugate: Unconjugated

Modification: Phosphorylated