Phospho-AKT1 (Thr450) Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-20804-60, E-AB-20804-120, E-AB-20804-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: Akt1 (phospho Thr450)
Target Synonym: AKT 1; AKT; AKT1; AKT1; MGC99656; PKB; PKB-ALPHA; PRKBA; Protein Kinase B Alpha; Protein kinase B; Proto-oncogene c-Akt; RAC Alpha; RAC; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Metabolism, Signal Transduction
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IHC-p, ELISA
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: P31749
Background: The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Concentration: 1 mg/mL
Immunogen: Synthesized peptide derived from human Akt1 around the phosphorylation site of Thr450
Buffer: PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:5000
Calculated MW: 56kDa
Observed MW: 55kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only