Product Description

Phospho-Artemis (Ser516) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16478-50, BZ16478-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q96SD1

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-P, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

Background: Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 78 kDa; Observed MW: 78 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma, using Phospho-Artemis antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval. Sample with blocking peptide on the right., Western blot analysis of Phospho-Artemis in HepG2 cells treated with EGF , using Phospho-Artemis antibody. The lane on the right is blocked with the Phospho- peptide.

Alternative Name: SCIDA; SNM1C; A-SCID; RS-SCID; DCLREC1C

Immunogen: The antiserum was produced against synthesized peptide derived from human Artemis around the phosphorylation site of Ser516. AA range:482-531

Conjugate: Unconjugated

Modification: Phosphorylated