Product Description

phospho-ASK1 (Thr845) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS65622-50, BS65622-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellμlar signal-regμlated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1, 374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, IHC

Alternate Names: ASK1 (phospho Thr845), Mitogen-activated protein kinase kinase kinase 5, MAPK/ERK kinase kinase 5, MEK kinase 5, MEKK 5, Apoptosis signal-regμlating kinase 1, ASK-1, MAP3K5, ASK1, MAPKKK5, MEKK5, M3K5_HUMAN.

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: KLH conjugated Synthesised phosphopeptide derived from human ASK1 around the phosphorylation site of Thr845.

Dilution: WB, 1:500 - 1:1000 IHC, 1:50 - 1:100

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: phospho-ASK1 (Thr845) Polyclonal Antibody detects endogenous levels of phospho-ASK1 protein only when phosphorylated at Thr845

Molecular Weight: ~ 155 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O35099

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only