Product Description

phospho-ASK1 (Thr845) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65622-50, BS65622-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: O35099

Host: Rabbit

Reactivity: Human, Mouse, Rat,

Applications: WB,

All Applications: WB=1:500-2000

Background: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: phospho-ASK1 (Thr845) Polyclonal Antibody detects endogenous levels of phospho-ASK1 protein only when phosphorylated at Thr845

Bioworld Molecular Weight: 154 kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Sample:
Lane 8: Cerebrum (Rat) Lysate at 40 ug
Lane 9: Stomach (Rat) Lysate at 40 ug
Primary: Anti-phospho-ASK1 (Thr845) at 1/1000 dilution, Sample:
Lane 1: Brain(Rat) Lysate at 30 ug
Lane 2: Heart(Rat) Lysate at 30 ug
Primary: Anti-phospho-ASK1(Thr845) at 1:200 dilution;

Alternative Name: ASK1 (phospho Thr845); Mitogen-activated protein kinase kinase kinase 5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; Apoptosis signal-regulating kinase 1; ASK-1; MAP3K5; ASK1; MAPKKK5; MEKK5; M3K5_HUMAN.

Immunogen: KLH conjugated Synthesised phosphopeptide derived from human ASK1 around the phosphorylation site of Thr845:TE(p-T)FT

Conjugate: Unconjugated

Modification: Phosphorylation