Product Description

Phospho-Bcl-2-S70 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79406-50, BS79406-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P10415

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 28kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of Jurkat and 293 cells, using Phospho-Bcl2 -S70 antibody at 1:1000 dilution. Jurkat cells were treated by serum-starvation overnight. 293 cells were treated by UV for 15-30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA., Immunofluorescence analysis of HeLa cells using BCL2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: Bcl-2; PPP1R50; BCL2

Immunogen: A synthetic phosphorylated peptide around S70 of human Bcl-2(NP_000624.2).

Conjugate: Unconjugated

Modification: Phosphorylated