Product Description

Phospho-BRCA1 (Ser1423) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BZ16480-50, BZ16480-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellμlar responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellμlar pathways such as DNA damage repair, ubiquitination and transcriptional regμlation to maintain genomic stability. Regμlates centrosomal microtubμle nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regμlation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regμlator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modμlatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regμlates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator (PubMed:20160719).

Category: Primary Antibody

Reactivity: Human, Rat

Host: Rabbit

Applications: WB, IHC-P, ELISA

Alternate Names: BRCA1, RNF53, Breast cancer type 1 susceptibility protein, RING finger protein 53

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: The antiserum was produced against synthesized peptide derived from human BRCA1 around the phosphorylation site of Ser1423. AA range:1391-1440

Dilution: WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

Purification: Affinity Purified

Specificity: IgG

Molecular Weight: Calcμlated MW: 208 kDa, Observed MW: 208 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P38398

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only