Phospho-Cardiac Troponin I (Ser22/Ser23) Polyclonal Antibody-BZ16417

Phospho-Cardiac Troponin I (Ser22/Ser23) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16417-50, BZ16417-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P23693

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB, IHC-P, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

Background: Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: tnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 24 kDa; Observed MW: 28 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-Cardiac Troponin I in SH-SY5Y lysates using Phospho-Cardiac Troponin I antibody., EnzymeLinked Immunosorbent Assay for Immunogen Phospho-peptide and NonPhospho-peptide, using TNNI3 antibody., Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle using TNNI3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right., Western blot analysis of Phospho-Cardiac Troponin I in mouse heart lysates using Phospho-Cardiac Troponin I antibody. The Lane on the right is blocked with the Phospho- peptide.

Alternative Name: TNNI3; TNNC1; Troponin I; cardiac muscle; Cardiac troponin I

Immunogen: The antiserum was produced against synthesized peptide derived from rat TNNI3 around the phosphorylation site of Ser22 and Ser23. AA range:5-54

Conjugate: Unconjugated

Modification: Phosphorylated