Product Description

Phospho-CDK1-T161 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6340-50, BS6340-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06493

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IP,

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IP,1:50 - 1:100

Background: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated Antibodies

Bioworld Molecular Weight: 34KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of HeLa, using Phospho-CDK1-T161 antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-CDK1-T161 pAb. Western blot was performed from the immunoprecipitate using Phospho-CDK1-T161 pAb at a dilution of 1:1000. HeLa cells were treated by EGF at 37℃ for 30 minutes after serum-starvation overnight.

Alternative Name: CDK1; CDC2; CDC28A; P34CDC2

Immunogen: A synthetic phosphorylated peptide around T161 of human CDK1(NP_001777.1).

Conjugate: Unconjugated

Modification: Phosphorylated