Product Description

Phospho-CFL1-Y139 Polyclonal Antibody

Catalogue Numbers:BS74141-50, BS74141-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P23528

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

All Applications: IHC,1:50 - 1:100

Background: The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: Phosphorylated

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-CFL1-Y139 Rabbit pAb at dilution of 1:50.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-CFL1-Y139 Rabbit pAb at dilution of 1:50.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded rat kidney using Phospho-CFL1-Y139 Rabbit pAb at dilution of 1:50.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Alternative Name: CFL1;CFL;HEL-S-15;cofilin;cofilin-1

Immunogen: A synthetic phosphorylated peptide around Y139 of human Phospho-CFL1-Y139(NP_005498.1).

Conjugate: Unconjugated

Modification: Phosphorylated