Phospho-Chk2 (Thr68) Polyclonal Antibody-BZ16375

Phospho-Chk2 (Thr68) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16375-50, BZ16375-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: O96017

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB,ICC/IF,IHC-P

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: These are known to be preferred sites for phosphorylation by ATM/ATR kinases. After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-Chk2 in Jurkat treated with UVLysates using Phospho- Chk2 antibody.

Bioworld Molecular Weight: Calculated MW: 61 kDa; Observed MW: 61 kDa

Specificity: IgG

Alternative Name: CHEK2; CDS1; CHK2; RAD53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2

Immunogen: The antiserum was produced against synthesized peptide derived from human Chk2 around the phosphorylation site of Thr68. AA range:35-84

Conjugate: Unconjugated

Modification: Phosphorylated