Product Description

phospho-CK II beta (Ser209) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65406-50, BS65406-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P67871

Host: Rabbit

Reactivity: Human, Mouse, Rat, Pig, Cow,

Applications: IHC-P

All Applications: IHC-P=1:100-500

Background: This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus.

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: phospho-CK II beta (Ser209) Polyclonal Antibody detects endogenous levels of CK II beta protein only when phosphorylated at S209.

Bioworld Molecular Weight: 24kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK II beta (Ser209)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C., Paraformaldehyde-fixed, paraffin embedded (Human ovarian cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK II beta (Ser209)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.

Alternative Name: Casein Kinase 2 beta (phospho S209); Casein Kinase 2 beta (p-S209); p-CSK2B(Ser209); phospho-CSK2B(Ser209); Casein kinase 2 beta polypeptide; casein kinase IIβ; Casein kinase II subunit beta; CK II beta; CK2B; CK2N; CSK2B; CSK2B_HUMAN; CSNK 2B; CSNK2B; G5A; MGC138222; MGC138224; Phosvitin; Protein G5a.

Immunogen: KLH conjugated synthesised phosphopeptide derived from human CK II beta around the phosphorylation site of Ser209:FK(p-S)PV

Conjugate: Unconjugated

Modification: Phosphorylation