Phospho-COT (Thr290) Polyclonal Antibody-BZ16504

Phospho-COT (Thr290) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16504-50, BZ16504-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P41279

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB,IHC-P,ICC/IF,ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: mitogen-activated protein kinase kinase kinase 8(MAP3K8) Homo sapiens This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-COT in 293Lysates treated with UV using Phospho-COT antibody. The lane on the right is blocked with the Phospho- peptide.

Bioworld Molecular Weight: Calculated MW: 53 kDa; Observed MW: 60 kDa

Specificity: IgG

Alternative Name: Mitogen-activated protein kinase kinase kinase 8; Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2

Immunogen: The antiserum was produced against synthesized peptide derived from human COT around the phosphorylation site of Thr290. AA range:256-305

Conjugate: Unconjugated

Modification: Phosphorylated