Product Description

Phospho-COT (Thr290) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BZ16504-50, BZ16504-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: mitogen-activated protein kinase kinase kinase 8(MAP3K8) Homo sapiens This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing resμlts in mμltiple transcript variants that encode the same protein.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC-P, ICC/IF, ELISA

Alternate Names: Mitogen-activated protein kinase kinase kinase 8, Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: The antiserum was produced against synthesized peptide derived from human COT around the phosphorylation site of Thr290. AA range:256-305

Dilution: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Purification: Affinity Purified

Specificity: IgG

Molecular Weight: Calcμlated MW: 53 kDa, Observed MW: 60 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P41279

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Research Use Only