Phospho-CRKL-Y207 Polyclonal Antibody-BS74184

Phospho-CRKL-Y207 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74184-50, BS74184-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P46109

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:100

Background: This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 38kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of K-562 cells, using Phospho-CRKL-Y207 antibody at 1:1000 dilution or CRKL antibody. K-562 cells were treated by CIP at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s., Immunohistochemistry of paraffin-embedded rat brain using Phospho-CRKL-Y207 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Alternative Name: CRKL

Immunogen: A synthetic phosphorylated peptide around Y207 of human CRKL(NP_005198.1).

Conjugate: Unconjugated

Modification: Phosphorylated