Product Description

Phospho-EEF2K-S366 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74189-50, BS74189-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O00418

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB,

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 110kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa cells, using Phospho-EEF2K-S366 antibody at 1:1000 dilution. Hela cell lysate were treated by CIP at 37℃ for 1 hour or treated by PMA/TPA for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 15s., Western blot analysis of extracts of various cell lines, using Phospho-EEF2K-S366 antibody at 1:1000 dilution.NIH/3T3 and C6 cells were treated by CIP at 37℃ for 1 hour or PMA/TPA at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Western blot analysis of extracts of HeLa cells, using Phospho-EEF2K-S366 pAb at 1:1000 dilution or EEF2K antibody. Hela cell lysate were treated by CIP at 37℃ for 1 hour or treated by PMA/TPA for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 15s.

Alternative Name: EEF2K; HSU93850; eEF-2K

Immunogen: A synthetic phosphorylated peptide around S366 of human EEF2K(NP_037434.1).

Conjugate: Unconjugated

Modification: Phosphorylated