Product Description

Phospho-EGFR (Tyr1092) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16408-50, BZ16408-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P00533

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

Background: EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. . A single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 134 kDa; Observed MW: 140-160 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-EGFR in various lysates using Phospho-EGFR antibody., Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma, using EGFR antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right., Western blot analysis of Phospho-EGFR in HUVEC lysates treated with EGF, using EGFR antibody. The Lane on the right is blocked with the Phospho- peptide.

Alternative Name: EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Immunogen: The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Tyr1092. AA range:1061-1110

Conjugate: Unconjugated

Modification: Phosphorylated