Product Description

Phospho-EGFR-Y1068 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79365-50, BS79365-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P00533

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IP,

All Applications: WB,1:1000 - 1:2000 | IP,1:50 - 1:100

Background: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 185kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa cells, using Phospho-EGFR-Y1068 pAb at 1:1000 dilution or EGFR antibody. HeLa cells were treated by EGF at 37℃ for 30 minutes after serum-starvation overnight.Hela cells were treated by ATP at 30℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunoprecipitation analysis of 200ug extracts of A-431 cells, using 3 ug Phospho-EGFR-Y1068 pAb. Western blot was performed from the immunoprecipitate using Phospho-EGFR-Y1068 pAb at a dilution of 1:1000. A-431 cells were treated by EGF at 37℃ for 30 minutes after serum-starvation overnight.

Alternative Name: EGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA

Immunogen: A synthetic phosphorylated peptide around Y1068 of human EGFR(NP_005219.2).

Conjugate: Unconjugated

Modification: Phosphorylated