Product Description

Phospho-eIF4E (Ser209) Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB10868-50, MB10868-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: eIF4E, a protein modμlates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regμlates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC-F, IHC-P, ICC/IF, IP

Alternate Names: EIF4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser209 of human eIF4E

Dilution: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Purification: Affinity Purified

Specificity: IgG

Molecular Weight: Calcμlated MW: 25 kDa, Observed MW: 25 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P06730

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Research Use Only