Product Description

Phospho-eIF4EBP1-S65 Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS6358-50, BS6358-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13541

Host: Rabbit

Reactivity: Human

Applications: WB, IP

All Applications: WB,1:500 - 1:2000 | IP,1:50 - 1:100

Background: This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293T cells, using Phospho-eIF4EBP1-S65 antibody at 1:1000 dilution. 293T cells were treated by IGF-1 at 37℃ for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Bioworld Molecular Weight: 20KDa

Specificity: Phosphorylated Antibodies

Alternative Name: 4E-BP1; 4EBP1; BP-1; PHAS-I; EIF4EBP1

Immunogen: A synthetic phosphorylated peptide around S65 of human Phospho-eIF4EBP1-S65(NP_004086.1).

Conjugate: Unconjugated

Modification: Phosphorylated