Product Description

Phospho-eNOS-S1177 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79371-50, BS79371-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29474

Host: Rabbit

Reactivity: Human

Applications: WB,

All Applications: WB,1:500 - 1:1000

Background: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 133kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of Jurkat cells, using Phospho-eNOS-S1177 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA., Western blot analysis of extracts of HeLa cells, using Phospho-NOS3-S1177 pAb at 1:1000 dilution or eNOS antibody. HeLa cells were treated by IGF-1 at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 3min.

Alternative Name: NOS3; ECNOS; eNOS

Immunogen: A synthetic phosphorylated peptide around S1177 of human eNOS(NP_000594.2).

Conjugate: Unconjugated

Modification: Phosphorylated