Phospho-ErbB 2 (Tyr1221/Tyr1222) Polyclonal Antibody-BZ16411

Phospho-ErbB 2 (Tyr1221/Tyr1222) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16411-50, BZ16411-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P04626

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, IP, , ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20 ELISA: 1/10000

Background: This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 138 kDa; Observed MW: 180 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-ErbB 2 in various lysates using Phospho-ErbB 2 antibody., Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma, using HER2 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right., Western blot analysis of Phospho-ErbB 2 in SKOV-3 lysates treated with EGF, using HER2 antibody. The Lane on the right is blocked with the Phospho- peptide.

Alternative Name: ERBB2; HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD antigen CD340

Immunogen: The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Tyr1221/Tyr1222. AA range:1191-1240

Conjugate: Unconjugated

Modification: Phosphorylated