Phospho-ERK1/2 (Thr202/Thr185) Monoclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: MB10417-50, MB10417-100
Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Swiss-Prot: P27361/P28482
Host: Rabbit
Reactivity: Human, Rat
Applications: WB, IP,
All Applications: WB: 1/500-1/1000 IP: 1/20
Background: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements.
Purification and Purity: Affinity Purified
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IgG
Bioworld Molecular Weight: Calculated MW: 44,42 kDa; Observed MW: 44,42 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Phospho-ERK1 /ERK2 in Hela, Hela+TPA, C6, C6+TPA lysates using Phospho-ERK1/2 antibody., Western blot analysis of Phospho-p44/42 MAPK in C6, Hela lysates using Phospho-p44/42 MAPK antibody.
Alternative Name: MAPK1/MAPK3
Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Thr202 of human ERK1
Conjugate: Unconjugated
Modification: Phosphorylated