Phospho-GSK3 beta (Ser9) Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-20886-60, E-AB-20886-120, E-AB-20886-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: GSK3β (phospho Ser9)
Target Synonym: Glycogen Synthase Kinase 3 Beta; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK-3 beta; GSK3B; GSK3B; GSK3beta isoform; Serine/threonine-protein kinase GSK3B
Research Areas: Cancer, Cardiovascular, Metabolism, Neuroscience, Signal Transduction, Stem Cells
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat
Application: WB, IHC-p, IF, ELISA
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: P49841
Background: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
Concentration: 1 mg/mL
Immunogen: Synthesized peptide derived from human GSK3β around the phosphorylation site of Ser9
Buffer: PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:100-1:300, ELISA 1:5000-1:20000
Calculated MW: 47kDa
Observed MW: 48kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only