Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Polyclonal Antibody-BS74139

Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74139-50, BS74139-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P56524/Q9UQL6/Q9UKV0

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB,1:500 - 1:1000 | IHC,1:50 - 1:100

Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 125kDa/140kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts from 293 cells untreated or treated with EGF using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA., Immunohistochemistry of paraffin-embedded human placenta using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse spleen using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded rat brain using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Alternative Name: HDAC4/HDAC5/HDAC9

Immunogen: A phospho specific peptide corresponding to residues surrounding S246 of humanHDAC4

Conjugate: Unconjugated

Modification: Phosphorylated