Phospho-Histone H2A-S129 Polyclonal Antibody-BS79470

Phospho-Histone H2A-S129 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79470-50, BS79470-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P04911

Host: Rabbit

Reactivity: Human, Rat

Applications: IHC

All Applications: IHC,1:50 - 1:200

Background: Core component of nucleosome which plays a central role in DNA double strand break (DSB) repair. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Immunogen: A synthetic phosphorylated peptide around S129 of saccharomyces cerevisiae Histone H2A(NP_010511.3?).

Conjugate: Unconjugated

Modification: Phosphorylated

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry of paraffin-embedded rat liver using Phospho-Histone H2A-S129 antibody at dilution of 1:100.Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-Histone H2A-S129 antibody at dilution of 1:100.Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.