Phospho-Histone H3 (S10) Rabbit Monoclonal Antibody-BS40010

Phospho-Histone H3 (S10) Rabbit Monoclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Sizes: 50µl, 100µl

Catalogue Numbers: BS40010-50, BS40010-100

Swiss-Prot: P68431

Host: Rabbit

Phospho-Histone H3 (S10) Rabbit Monoclonal Antibody-BS40010

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse, Rat

Applications: WB, IHC ICC/IF, IP,

All Applications: WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50

Background: Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.

Purification and Purity: Affinity-chromatography

Specificity: Phospho-Histone H3 (S10) Antibody detects endogenous levels of total Phospho-Histone H3 (S10)

Bioworld Molecular Weight: ~ 18 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Histone H3 (Phospho-Ser10) Rabbit monoclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate (30ug)
Lane 2: HEK293T treated with Calyculin A (50 nM,30 minutes) whole cell lysate (30ug)
Lane 3: Hela whole cell lysate (30ug)
Lane 4: The Lung tissue lysate of Mouse (30ug)
Lane 5: The Heart tissue lysate of Rat (30ug), Immunohistochemical analysis of paraffin-embedded mouse liver, using Phospho-Histone H3 (S10) Antibody.

Alternative Name: H3 histone, family 3A; H3 histone, family 3B (H3.3B); H3.3A; H3.3B; H33; H3F3; H3F3A; H3F3B; Histone H3.3;

Immunogen: A synthesized peptide derived from human Phospho-Histone H3 (S10)

Modification: Phosphorylated

Conjugate: Unconjugated