Phospho-Histone H3-T3 Polyclonal Antibody-BS79445

Phospho-Histone H3-T3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79445-50, BS79445-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P68431

Host: Rabbit

Reactivity: Human, Mouse, Rat, Other (Wide Range)

Applications: WB,

All Applications: WB,1:500 - 1:2000

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 17KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-T3 pAb at 1:1000 dilution.C6 and HeLa cells were treated by Nocodazole at 37℃ for 20 hours.C6 and NIH/3T3 cells were treated by CIP at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-T3 pAb at 1:1000 dilution or Histone H3 antibody. Both 293T cells and Raw264.7 cells were treated by Nocodazole at 37℃ for 16 hours.C6 cells were treated by Nocodazole at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.

Alternative Name: H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A

Immunogen: A synthetic phosphorylated peptide around T3 of human HIST1H3A(NP_003520.1).

Conjugate: Unconjugated

Modification: Phosphorylated