Phospho-Histone H3 (Thr3) Polyclonal Antibody-BZ16552

Phospho-Histone H3 (Thr3) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16552-50, BZ16552-100

Product: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Swiss-Prot: P84243

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-P, ICC/IF, FC

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 FC: 1/50-1/100

Background: H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 15 kDa; Observed MW: 15 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-Histone H3 in HeLa lysates treated with FBS + Calyculin A using Phospho-Histone H3 antibody., Immunohistochemistry analysis of paraffin-embedded Human tonsil using Phospho-Histone H3 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Alternative Name: H3F3; H3.3A

Immunogen: A synthesized peptide derived from human Phospho-Histone H3 (T3)

Conjugate: Unconjugated

Modification: Phosphorylated