Phospho-HP1 gamma/CBX3-S83 Polyclonal Antibody-BS74177

Phospho-HP1 gamma/CBX3-S83 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74177-50, BS74177-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13185

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB,

All Applications: WB,1:500 - 1:2000

Background: At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 22kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of HeLa cells, using Phospho-HP1 gamma/CBX3-S83 pAb at 1:1000 dilution or HP1 gamma/CBX3 antibody. HeLa cells were treated by Paclitaxel at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 60s., Western blot analysis of extracts of NIH/3T3 cells, using Phospho-HP1 gamma/CBX3-S83 antibody at 1:1000 dilution.NIH/3T3 cells were treated by Paclitaxel at 37℃ for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s.

Alternative Name: CBX3; HECH; HP1-GAMMA; HP1Hs-gamma

Immunogen: A synthetic phosphorylated peptide around S83 of human HP1 gamma/CBX3(NP_009207.2).

Conjugate: Unconjugated

Modification: Phosphorylated