Phospho-IGF-IReceptorβ(Tyr1135/1136)/InsulinReceptorβ(Tyr1150/1151) Polyclonal Antibody-BS74229

Phospho-IGF-IReceptorβ(Tyr1135/1136)/InsulinReceptorβ(Tyr1150/1151) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74229-50, BS74229-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06213/P08069

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants.This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of HeLa, using Phospho-IGF-IReceptorβ/InsulinReceptorβ antibody at 1:2540 dilution. HeLa cells were treated by IGF-1 at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.

Bioworld Molecular Weight: 102KDa

Specificity: Phosphorylated

Alternative Name: HHF5; CD220; IGFR; CD221; IGFIR; JTK13

Immunogen: A synthetic phosphorylated peptide around IReceptorβ(Tyr1135/1136)/InsulinReceptorβ(Tyr1150/1151) of human(Tyr1150/1151) of human IGF.

Conjugate: Unconjugated

Modification: Phosphorylated