Phospho-IkB alpha (Ser32/S36) Polyclonal Antibody
Sizes: 60μL, 120μL, 200μL
Catalogue Numbers: E-AB-20911-60, E-AB-20911-120, E-AB-20911-200
Citations, Manuals and MSDS Available upon request.
Abbreviation: IκB-α (phospho Ser32/36)
Target Synonym: I kappa B alpha; I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappa-B inhibitor alpha; NFKBI; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Immunology, Signal Transduction
Conjugation: Unconjugated
Host: Rabbit
Species Reactivity: Human, Mouse, Rat, Monkey
Application: WB, IHC-p, IF, ELISA
Isotype: IgG
Clonality: Polyclonal
UNIProt ID: P25963
Background: Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Concentration: 1 mg/mL
Immunogen: Synthesized peptide derived from human IκB-α around the phosphorylation site of Ser32/36
Buffer: PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Purification Method: Affinity purification
Dilution: WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:10000
Calculated MW: 36kDa
Observed MW: 34kDa
Storage: -20°C/One year. Avoid freeze / thaw cycles.
Shipping: Ice bag
Research Use Only