Product Description

Phospho-IRS1 (Ser636) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16044-50, BZ16044-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P35568

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit .

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 132 kDa; Observed MW: 150 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-IRS1 in SH-SY5Y lysates using Phospho-IRS1 antibody., Immunohistochemistry analysis of paraffin-embedded Human brain using Phospho-IRS1 antibody. High-pressure and temperature Tris-EDTA pH 8. 0 was used for antigen retrieval. Sample with blocking peptide on the right.

Alternative Name: IRS1; Insulin receptor substrate 1; IRS-1

Immunogen: The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser636. AA range:603-652

Conjugate: Unconjugated

Modification: Phosphorylated