Product Description

Phospho-ITK (Tyr512) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16331-50, BZ16331-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q08881

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC-P, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

Background: Interleukin-2 inducible T-cell kinase (Itk, Emt or Tsk) is a member of the non-receptor protein tyrosine kinases. Family members of Itk include Tec, Btk, Rlk and Bmx and are all defined by a common structure: an amino-terminal PH domain, a Tec-homology domain and a SH3 and SH2 domain followed by a carboxy-terminal kinase domain. Tec, Rlk and Itk are expressed in T cells and activated in response to T cell receptor (TCR) engagement.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 72 kDa; Observed MW: 72 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Phospho-ITK in 293 lysates using Phospho-ITK antibody., Immunohistochemistry analysis of paraffin-embedded Human testis using Phospho-ITK antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval. Sample with blocking peptide on the right.

Alternative Name: ITK; EMT; LYK; Tyrosine-protein kinase ITK/TSK; Interleukin-2-inducible T-cell kinase; IL-2-inducible T-cell kinase; Kinase EMT; T-cell-specific kinase; Tyrosine-protein kinase Lyk

Immunogen: Synthetic peptide of human ITK

Conjugate: Unconjugated

Modification: Phosphorylated