Phospho-Jak1-Y1022/1023 Polyclonal Antibody-BS79393

Phospho-Jak1-Y1022/1023 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79393-50, BS79393-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P23458

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a membrane protein that is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The encoded kinase phosphorylates STAT proteins (signal transducers and activators of transcription) and plays a key role in interferon-alpha/beta and interferon-gamma signal transduction. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 130KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of RAW264.7 cells, using Phospho-Jak1-Y1022/1023 antibody at 1:1000 dilution.RAW264.7 cells were treated by Pervanadate at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s., Immunofluorescence analysis of NIH/3T3 cells using Phospho-Jak1-Y1022/1023 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: JAK1; JAK1A; JAK1B; JTK3

Immunogen: A synthetic phosphorylated peptide around Y1022 & Y1023 of human JAK1(NP_002218.2).

Conjugate: Unconjugated

Modification: Phosphorylated