Product Description

Phospho-JAK2-Y1007/1008 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS4109-50, BS4109-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60674

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB,1:500 - 1:1000 | IHC,1:50 - 1:200

Background: This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated Antibodies

Bioworld Molecular Weight: 125KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of C6, using Phospho-JAK2-Y1007/1008 antibody at 1:1761.5 dilution.C6 cells were treated by Pervanadate at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s., Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-JAK2-Y1007/1008 antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse brain using Phospho-JAK2-Y1007/1008 antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded rat spleen using Phospho-JAK2-Y1007/1008 antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Alternative Name: JAK2; JTK10; THCYT3

Immunogen: A synthetic phosphorylated peptide around Y1007 & Y1008 of human JAK2(NP_004963.1?).

Conjugate: Unconjugated

Modification: Phosphorylated