Phospho-Lck-Y505 Polyclonal Antibody-BS79364

Phospho-Lck-Y505 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79364-50, BS79364-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06239

Host: Rabbit

Reactivity: Human

Applications: WB, IP,

All Applications: WB,1:500 - 1:2000 | IP,1:50 - 1:100

Background: This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants encoding different isoforms have been described.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated

Bioworld Molecular Weight: 56KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of Jurkat cells, using Phospho-Lck-Y505 antibody at 1:1000 dilution.Jurkat cells were treated by Hydrogen Peroxide at 37℃ for 2 minutes after serum-starvation overnight.Jurkat cells were treated by Pervanadate at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 1s., Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-Lck-Y505 pAb. Western blot was performed from the immunoprecipitate using Phospho-Lck-Y505 pAb at a dilution of 1:1000. Jurkat cells were treated by Hydrogen Peroxide at 37℃ for 2 minutes after serum-starvation overnight.

Alternative Name: LCK; IMD22; LSK; YT16; p56lck; pp58lck

Immunogen: A synthetic phosphorylated peptide around Y505 of human Lck(NP_001036236.1).

Conjugate: Unconjugated

Modification: Phosphorylated