Phospho-NF-kB p65/RelA-S536 Polyclonal Antibody-BS66164

Phospho-NF-kB p65/RelA-S536 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS66164-50, BS66164-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q04206

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB,

All Applications: WB,1:1000 - 1:7000

Background: NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Phosphorylated Antibodies

Bioworld Molecular Weight: 65KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Hela, using Phospho-NF-kB p65/RelA-S536 antibody at 1:7000 dilution.HeLa cells were treated by TNF-α and Calyculin A at 37℃ for 10 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Western blot analysis of NIH/3T3, using Phospho-NF-kB p65/RelA-S536 antibody at 1:7000 dilution.NIH/3T3 cells were treated by TNF-α at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Western blot analysis of C6, using Phospho-NF-kB p65/RelA-S536 antibody at 1:7000 dilution.NIH/3T3 cells were treated by TNF-α at 37℃ for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Alternative Name: NFKB3; p65; NF-kB p65; RELA; CMCU

Immunogen: A synthetic phosphorylated peptide around S536 of human RelA(NP_068810.3).

Conjugate: Unconjugated

Modification: Phosphorylated