Product Description

Phospho-NPM1-T199 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79441-50, BS79441-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06748

Host: Rabbit

Reactivity: Human, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Phospho-NPM1-T199 pAb at 1:1000 dilution or NPM1 antibody. HeLa cells were treated by nocodazole at 37℃ for 20 hours. PC-12 cells were treated by Nocodazole at 37℃ for 16 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Bioworld Molecular Weight: 38kDa

Specificity: Phosphorylated

Alternative Name: B23; NPM; NPM1

Immunogen: A synthetic phosphorylated peptide around T199 of human NPM1(NP_001032827.1).

Conjugate: Unconjugated

Modification: Phosphorylated